Emory Chemical Biology Discovery Center


Emory Chemical Biology Discovery Center

Principal Investigator: Raymond Dingledine

The Emory Chemical Biology Center has the capability to adapt and optimize all target-based and phenotypic assays selected by the MLSCN but has identified protein-protein interactions for small molecule discovery as our Center’s theme. With two general screening platforms established, the center is able to perform both HTS and HCS using a variety of in vitro biochemical assays, cell reporter assays, and cell phenotype-based assays. In particular, this center is experienced in assays for monitoring protein-protein interactions and enzyme activities with fluorescence-based assays, including fluorescence intensity, fluorescence polarization, and FRET. Examples of assays that are within our center’s capacity include protein-protein interaction (FI, FP, FRET, AlphaScreen), enzyme assays (FI, FP, and other coupled assays), receptor-ligand interaction (FI, FRET, Ca2+ imaging), reporter assays (luciferase, GFP, etc), viability assays and protein translocation (e.g., receptor internalization and membrane & nuclear localization).

Emory Capabilities and Technologies

Assay Formats Screening Capabilities Biologicial Expertise
Fluorescence intensity High Content Assays Protein-protein interactions
Fluorescence polarization Variety of in vitro biochemical assays Enzyme Assays
FRET Cell reporter assays Receptor-ligand interaction
Luminescence (including Alphascreen) Phenotype-based assays Reporter Assays
Image-based (HCS) Fluorescence-based assays Viability Assays
Absorbance Luminescence-based assays Protein Translocation
  Virtual screening  

For inquiries about assay development at the The Emory Chemical Biology Center, please contact the center representative listed below in conjunction with visiting the Center’s Web site.

See also: Enabling PI-Center Collaboration Document

For more information about The Emory Chemical Biology Center, see below: