MLSCN Center at Columbia University

Principal Investigator: James Rothman

The strengths and experience of the MLSCN Center at Columbia University are in cell biology, high content/high resolution automated cellular imaging and image analysis, and phenotypic assay design and implementation. The main imaging platform of the center at Columbia University is the INCell Analyzer 3000 (GE Healthcare), a state of the art high throughput cell imaging system. The INCell Analyzer 3000 uses three laser lines for excitation: a Krypton laser (647 nm) and an Argon laser (364 nm and 488 nm). Three fluorescence channels can be recorded by three independent high speed 12 bit CCD cameras. Emitted light in the wavelength range from 420 nm to 720 nm can be captured. Being connected to a Kendro Plate Hotel and a Mitsubishi robotic arm, the system can image and analyze 222 plates (96/384 well) without supervision. Depending on the specific assay up to 50,000 wells can be processed per day. Image analysis is done at high speed on the fly. A whole array of different image analysis modules is available. This imaging system enables the center to screen and analyze a very broad variety of assays monitoring a wide spectrum of biological processes.

Columbia Capabilities and Technologies

For inquiries about assay development at The MLSCN Center at Columbia University, please contact the center representative listed below.

• MLSCN Center at Columbia University
  • Please contact: James Rothman, Ph.D.

See also: Enabling PI-Center Collaboration Document