New Mexico Molecular Libraries Screening Center
New Mexico Molecular Libraries Screening Center
Principal Investigator: Larry Sklar
The New Mexico Molecular Libraries Screening Center has developed innovative flow cytometric tools for discovery research that enable homogeneous analysis of ligand binding and protein-protein interaction, HT sample handling, high content analysis, and real-time measurements of cell response. Our experience indicates that virtually any molecular assembly or cell response can be displayed in a format compatible with flow cytometry. Moreover, by creating a suspension array of particles, assays and responses can be highly multiplexed or performed on complex cell populations without loss of throughput. Our novel sampling approach (HyperCyt ?) makes flow cytometry an attractive platform for drug discovery, proteomics, and real-time analysis of molecular interactions. Flow cytometry is particularly convenient for alternately assessing both cellular and molecular activities of small molecules. To our knowledge, there is no single competing technology that offers the versatility of flow cytometry for Molecular Library Initiative screening or that has the potential of being available to such a large number of laboratories that house flow cytometers (20,000 world-wide). Our team brings together expertise that spans biomedical, biophysical, chemical, computational, instrumentation and engineering disciplines. We are particularly interested in enhancing the overall discovery process through the integration of physical screening and computational tools that include virtual screening, cheminformatics, and data mining.
New Mexico Capabilities and Technologies
| Assay Formats | Screening Capabilities | Biologicial Expertise |
|---|---|---|
| High-throughput flow cytometry | Cell-based Assays | Protein-protein interactions |
| Solution and Suspension based absorbance, fluorescence steady-state and lifetime | Bead-based Assays | Protein-nucleic acid interactions |
| Fluorescence and phase-contrast microscopy | Informatics (virtual screening, clustering and in silico profiling) | Kinase assays |
| Plate Based fluorescence, absorbance, polarization, and luminescence | Multiparametric high content analysis | GPCRs |
| Multiparametric multiplex analysis | Integrins and adhesion molecules | |
| High throughput analysis of compound solubility | Proteases/proteasome | |
| High Throughput and Multiparametric analysis of compound fluorescence (3 colors of excitation, 9 colors of emission) | Cell cycle | |
| Apoptosis | ||
For inquiries about assay development at a particular The New Mexico Molecular Libraries Screening Center, please contact the center representative listed in conjunction with visiting the Center’s Web site.
- New Mexico Molecular Libraries Screening Center
- Please contact: Larry Sklar, Ph.D.
