San Diego Center for Chemical Genomics
San Diego Center for Chemical Genomics
Principal Investigator: John Reed
The San Diego Center for Chemical Genomics (SDCCG) has broad expertise in biochemistry and the ability to run almost any assay type. Specific biological themes include targets involved in regulating cell death, using a variety of biochemical and cell-based assays, with particular emphasis on kinases, phosphatases and proteases. Another area of expertise is in phenotypic assays for stem cell differentiation, using fluorescent reporters. Two main technological themes are incorporated into the SDCCG. First, the Center has special expertise in high-throughput microscopy as a tool for performing high-content, cell-based screens where cellular phenotypes drive compound selection in an unbiased manner. Second, the Center is unique across the network in having the capability to perform NMR-based small-molecule screening and optimization. NMR-based methods are exceptionally valuable when investigating molecular targets that are not easily tractable by other methods, such as protein-protein interactions and protein targets that cannot be formatted for the classical HTS environment.
Burnham Capabilities and Technologies
| Assay Formats | Screening Capabilities | Biologicial Expertise |
|---|---|---|
| Absorbance | Biochemical Assay Screening | Cell Death (Apoptosis, Necrosis etc.) |
| Luminescence | Cell-Based Assay Screening | Cell Differentiation (phenotypic assays for stem cells using fluorescent reporters) |
| Fluorescence Intensity | High-Content Cell-Based Assay Screening | Cell Proliferation |
| Fluorescence Polarization | High Throughput Microscopy/Cell Imaging | Cell Motility and Invasion |
| Time Resolved Fluorescence | Cell Image Analysis | Cell Morphology |
| FRET | Image Algorithm Development | Cytotoxicity Assays |
| TR-FRET | Bacterial, Baculovirus and Mammalian Recombinant Protein Expression Systems | Enzyme Assays |
| Heterogeneous Assay Formats (ex: ELISA) | Lentivirus Technology | Kinase Assays |
| NMR | Cell Reporter Assays | Phosphatase Assays |
| Transmitted Light | Cell Line Generation | Protease Assays |
| NMR-Based Screening | ER Stress/Chemical Chaperone Assays | |
| Virtual Library Design/Screening | Protein Translocation Assays | |
| In Silico Profiling | Integrins, Cadherins and Other Cell surface Protein Assays | |
| Protein-protein Interaction Assays | ||
| Cardiac myocyte Assays | ||
| Pancreatic beta-cell Assays | ||
| Neuroregeneration Assays | ||
| Promoter/Reporter Assays | ||
| Actins, Microtubulus and Microfilament Structures | ||
| Cancer | ||
| Diabetes | ||
| Inflammation and Infectious Diseases | ||
| Nucleic Acid-based Targets | ||
| Functional Genomics/cDNA/siRNA Profiling | ||
| PK/ADME/Toxicity Profiling |
For inquiries about assay development at The San Diego Center for Chemical Genomics, please contact the center representative listed in conjunction with visiting the Center’s Web site.
- San Diego Center for Chemical Genomics
- Please contact: Nicholas Cosford, Ph.D.
See also: Enabling PI-Center Collaboration Document
For further information about The San Diego Center for Chemical Genomics, see below:
- April 25, 2007 - In less than a year, the SDCCG has surpassed the 1 Million mark for screening data submitted to PubChem (see current summary).
- Screening Equipment
- To Contact the SDCCG
