Scripps Research Institute Molecular Screening Center
Scripps Research Institute Molecular Screening Center
Principal Investigator: Hugh Rosen
The Scripps Research Institute Molecular Screening Center spans the Scripps campuses in La Jolla and West Palm Beach. Scripps has brought together an integrated combination of infrastructure, people and technologies that can support the identification of proof-of-concept small molecules in the academic setting. By this we mean chemical probes of adequate potency, selectivity, physical properties and stability to show robust activities in cell-based assays and in vivo, allowing pre-competitive advancement to fields of breaking biology. The center is equipped with a fully automated screening system, Kalypsys Screening System, with plate hotels and incubators, 200 nL to 20 uL volume dispensing 1536-well aspiration, and 1536 pintool head for compound delivery for uHTS screening of larger compound decks. Plate Readers and detectors include ViewLux CCD-based plate reader multimode for TRF,FP, FI, FRET, luminescence, absorbance. Perkin Elmer Envsion, AlphaScreen and Cell imaging platform. This is vertically integrated with enterprise-scale data management and cheminformatics, high throughput LC_MS compound quality assurance and rodent pharmacokinetics, and facilties for downstream synthetic follow-up by modular, library or linear chemical approaches. The goal of the Scripps Center is the rapid, collaborative publication of interesting compounds that advance the understanding of biological problems, or illuminate new nodal control points in physiology by short-term chemical perturbation.
Scripps Capabilities and Technologies
| Assay Formats | Screening Capabilities | Biologicial Expertise |
|---|---|---|
| Fluorescence Intensity | Cell Imaging | GPCRs |
| Fluorescence Polarization | Fluorescent Microscopy | Receptor-ligand interaction |
| TRF | Protein-protein interactions | |
| FRET | Enzyme Assays | |
| Luminescence | Ion Channels | |
| Absorbance | Reporter Assays | |
| AlphaScreen | Viability Assays | |
| FITC | Protein Translocation | |
| GFP |
For inquiries about assay development at a The Scripps Research Institute Molecular Screening Center, please contact the center representatives listed in conjunction with visiting the Center’s Web site.
- Scripps Research Institute Molecular Screening Center
- Please contact: Steven Brown Ph.D. (Scripps, La Jolla, CA)
- Please contact: Peter Hodder, Ph.D. (Scripps, Jupiter, Florida)
For further information about the Scripps Research Institute Molecular Screening Center, see below:
